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rabbit polyclonal anti alix  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit polyclonal anti alix
    Quantitative capillary western blot analysis of the proteins derived from human EVs Human EVs were isolated after treatment of human blood with vehicle or with 2 nM Stx2a in the absence or in the presence of 0.01 μg/mL NAB815 and their proteins extracted as described in . (A) Representative WES of the analyzed antigens (Alix, CD45, and CD42a) and associated proteins (Stx2a) is shown. (B–E) Data obtained with different human donors ( n = 4) represent the percentage (mean ± SD) of the quantitative determinations of the different antigens with respect to controls (C–E) or Stx2a (B). (F–H) Data obtained with different human donors ( n = 4) are expressed as percentage (mean ± SD) of the stimulation in the presence of Stx2a that was set at 100%. Different primary antibodies were used: rabbit <t>polyclonal</t> anti-Alix 1:50 (Novus Biological) as an EV marker, mouse monoclonal anti-CD45 1:250 (BD Transduction Laboratories) as a leukocyte marker, rabbit polyclonal anti-CD42a 1:10 (GeneTex) as a platelet marker, and rabbit polyclonal anti-Stx2a 1:50 (Dr. Stefano Morabito, ISS Rome) to detect the toxin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (two-tailed unpaired t test). See also .
    Rabbit Polyclonal Anti Alix, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+alix/pmc12309968-190-5-9?v=Novus+Biologicals
    Average 93 stars, based on 2 article reviews
    rabbit polyclonal anti alix - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "An antibiotic derivative as a new potential tool in the prevention of hemolytic uremic syndrome"

    Article Title: An antibiotic derivative as a new potential tool in the prevention of hemolytic uremic syndrome

    Journal: iScience

    doi: 10.1016/j.isci.2025.113076

    Quantitative capillary western blot analysis of the proteins derived from human EVs Human EVs were isolated after treatment of human blood with vehicle or with 2 nM Stx2a in the absence or in the presence of 0.01 μg/mL NAB815 and their proteins extracted as described in . (A) Representative WES of the analyzed antigens (Alix, CD45, and CD42a) and associated proteins (Stx2a) is shown. (B–E) Data obtained with different human donors ( n = 4) represent the percentage (mean ± SD) of the quantitative determinations of the different antigens with respect to controls (C–E) or Stx2a (B). (F–H) Data obtained with different human donors ( n = 4) are expressed as percentage (mean ± SD) of the stimulation in the presence of Stx2a that was set at 100%. Different primary antibodies were used: rabbit polyclonal anti-Alix 1:50 (Novus Biological) as an EV marker, mouse monoclonal anti-CD45 1:250 (BD Transduction Laboratories) as a leukocyte marker, rabbit polyclonal anti-CD42a 1:10 (GeneTex) as a platelet marker, and rabbit polyclonal anti-Stx2a 1:50 (Dr. Stefano Morabito, ISS Rome) to detect the toxin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (two-tailed unpaired t test). See also .
    Figure Legend Snippet: Quantitative capillary western blot analysis of the proteins derived from human EVs Human EVs were isolated after treatment of human blood with vehicle or with 2 nM Stx2a in the absence or in the presence of 0.01 μg/mL NAB815 and their proteins extracted as described in . (A) Representative WES of the analyzed antigens (Alix, CD45, and CD42a) and associated proteins (Stx2a) is shown. (B–E) Data obtained with different human donors ( n = 4) represent the percentage (mean ± SD) of the quantitative determinations of the different antigens with respect to controls (C–E) or Stx2a (B). (F–H) Data obtained with different human donors ( n = 4) are expressed as percentage (mean ± SD) of the stimulation in the presence of Stx2a that was set at 100%. Different primary antibodies were used: rabbit polyclonal anti-Alix 1:50 (Novus Biological) as an EV marker, mouse monoclonal anti-CD45 1:250 (BD Transduction Laboratories) as a leukocyte marker, rabbit polyclonal anti-CD42a 1:10 (GeneTex) as a platelet marker, and rabbit polyclonal anti-Stx2a 1:50 (Dr. Stefano Morabito, ISS Rome) to detect the toxin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (two-tailed unpaired t test). See also .

    Techniques Used: Western Blot, Derivative Assay, Isolation, Marker, Transduction, Two Tailed Test



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    Quantitative capillary western blot analysis of the proteins derived from human EVs Human EVs were isolated after treatment of human blood with vehicle or with 2 nM Stx2a in the absence or in the presence of 0.01 μg/mL NAB815 and their proteins extracted as described in . (A) Representative WES of the analyzed antigens (Alix, CD45, and CD42a) and associated proteins (Stx2a) is shown. (B–E) Data obtained with different human donors ( n = 4) represent the percentage (mean ± SD) of the quantitative determinations of the different antigens with respect to controls (C–E) or Stx2a (B). (F–H) Data obtained with different human donors ( n = 4) are expressed as percentage (mean ± SD) of the stimulation in the presence of Stx2a that was set at 100%. Different primary antibodies were used: rabbit <t>polyclonal</t> anti-Alix 1:50 (Novus Biological) as an EV marker, mouse monoclonal anti-CD45 1:250 (BD Transduction Laboratories) as a leukocyte marker, rabbit polyclonal anti-CD42a 1:10 (GeneTex) as a platelet marker, and rabbit polyclonal anti-Stx2a 1:50 (Dr. Stefano Morabito, ISS Rome) to detect the toxin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (two-tailed unpaired t test). See also .
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    Image Search Results


    Quantitative capillary western blot analysis of the proteins derived from human EVs Human EVs were isolated after treatment of human blood with vehicle or with 2 nM Stx2a in the absence or in the presence of 0.01 μg/mL NAB815 and their proteins extracted as described in . (A) Representative WES of the analyzed antigens (Alix, CD45, and CD42a) and associated proteins (Stx2a) is shown. (B–E) Data obtained with different human donors ( n = 4) represent the percentage (mean ± SD) of the quantitative determinations of the different antigens with respect to controls (C–E) or Stx2a (B). (F–H) Data obtained with different human donors ( n = 4) are expressed as percentage (mean ± SD) of the stimulation in the presence of Stx2a that was set at 100%. Different primary antibodies were used: rabbit polyclonal anti-Alix 1:50 (Novus Biological) as an EV marker, mouse monoclonal anti-CD45 1:250 (BD Transduction Laboratories) as a leukocyte marker, rabbit polyclonal anti-CD42a 1:10 (GeneTex) as a platelet marker, and rabbit polyclonal anti-Stx2a 1:50 (Dr. Stefano Morabito, ISS Rome) to detect the toxin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (two-tailed unpaired t test). See also .

    Journal: iScience

    Article Title: An antibiotic derivative as a new potential tool in the prevention of hemolytic uremic syndrome

    doi: 10.1016/j.isci.2025.113076

    Figure Lengend Snippet: Quantitative capillary western blot analysis of the proteins derived from human EVs Human EVs were isolated after treatment of human blood with vehicle or with 2 nM Stx2a in the absence or in the presence of 0.01 μg/mL NAB815 and their proteins extracted as described in . (A) Representative WES of the analyzed antigens (Alix, CD45, and CD42a) and associated proteins (Stx2a) is shown. (B–E) Data obtained with different human donors ( n = 4) represent the percentage (mean ± SD) of the quantitative determinations of the different antigens with respect to controls (C–E) or Stx2a (B). (F–H) Data obtained with different human donors ( n = 4) are expressed as percentage (mean ± SD) of the stimulation in the presence of Stx2a that was set at 100%. Different primary antibodies were used: rabbit polyclonal anti-Alix 1:50 (Novus Biological) as an EV marker, mouse monoclonal anti-CD45 1:250 (BD Transduction Laboratories) as a leukocyte marker, rabbit polyclonal anti-CD42a 1:10 (GeneTex) as a platelet marker, and rabbit polyclonal anti-Stx2a 1:50 (Dr. Stefano Morabito, ISS Rome) to detect the toxin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (two-tailed unpaired t test). See also .

    Article Snippet: Different primary antibodies were used: rabbit polyclonal anti-Alix 1:50 (Novus Biological) as an EV marker, mouse monoclonal anti-CD45 1:250 (BD Transduction Laboratories) as a leukocyte marker, rabbit polyclonal anti-CD42a 1:10 (GeneTex) as a platelet marker, and rabbit polyclonal anti-Stx2a 1:50 (Dr. Stefano Morabito, ISS Rome) to detect the toxin.

    Techniques: Western Blot, Derivative Assay, Isolation, Marker, Transduction, Two Tailed Test